Construction of Multidrug-Sensitive Yeast with High Sporulation Efficiency

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Author(s)

Abstract

Budding yeast is often used in chemical genetics for screening, target identification, and compound verification, but its high-level drug resistance has made the analysis of compounds difficult. Here we report the construction of 12geneΔ0HSR, a strain that lacks eight efflux pumps located on the plasma membrane and four transcription factors involved in expression of efflux pumps, and contains the <I>RME1(ins-308A)</I> mutation. This strain retained sufficient transformation, mating, and sporulation efficiency for genetic analysis in addition to hypersensitivity against several compounds. 12geneΔ0HSR is a useful tool for chemical biology, not only in chemical screening but in target identification and verification of bioactive compounds.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 75(8), 1588-1593, 2011-08-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  35

Codes

  • NII Article ID (NAID)
    10029591836
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09168451
  • NDL Article ID
    11222557
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  NDL  J-STAGE 
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