Synthesis of Stable Isotope-Labeled Precursors for the Biosyntheses of Capsaicinoids, Capsinoids, and Capsiconinoids

• KOBATA Kenji

  Graduate School of Nutritional and Environmental Sciences and Global COE Program, University of Shizuoka Faculty of Pharmaceutical Sciences, Josai University

• MIMURA Makoto

  Graduate School of Nutritional and Environmental Sciences and Global COE Program, University of Shizuoka

• SUGAWARA Mai

  Graduate School of Nutritional and Environmental Sciences and Global COE Program, University of Shizuoka

• WATANABE Tatsuo

  Graduate School of Nutritional and Environmental Sciences and Global COE Program, University of Shizuoka

抄録

Stable isotope-labeled precursors were synthesized for an analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) to elucidate the biosynthetic flow of capsaicinoids, capsinoids, and capsiconinoids. [1′-¹³C][5-²H]-Vanillin was prepared by the condensation of guaiacol with [¹³C]-chloroform and a D₂O treatment. Labeled vanillylamine, vanillyl alcohol, ferulic acid, and coniferyl alcohol were prepared from the labeled vanillin. The labeled vanillylamine was converted to labeled capsaicinoid in a crude enzyme solution extracted from pungent Capsicum fruits.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 75 (8), 1611-1614, 2011

  公益社団法人 日本農芸化学会

キーワード

• stable isotope
• capsaicinoid
• capsinoid
• capsaicin synthase
• liquid chromatography-tandem mass spectrometry (LC-MS/MS)

詳細情報

• CRID: 1390001206479878272
• NII論文ID: 10029591963
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.110187
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 11222633
• PubMed: 21821932
• Web Site: http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-bbb.110187
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11222633
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110187
• 本文言語コード: en
• データソース種別:
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