Localization of a Novel RNA-binding Protein, SKIV2L2, to the Nucleus in the Round Spermatids of Mice

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Abstract

In our previous study (Kawashima <i>et al., Biol Reprod</i> 2009; 80: 1293-1304), we suggested that the first cycle of spermatogenesis recovered from busulfan-induced temporary arrest was a good model system to analyze the proteins expressed at the specific stages of spermatogenesis in the mouse, and this has been confirmed in the present paper. Namely, six-week-old mice were injected with busulfan at 20 mg/kg body weight. The germ cells except for the undifferentiated spermatogonia disappeared by 32 days after injection. The surviving spermatogonia started to proliferate, and spermatogenesis was entirely recovered about 77 days after injection. By proteome analysis of the busulfan-treated testis during the process of recovery of spermatogenesis, we identified a protein that was expected to be expressed in the spermatogenic cells as Superkiller viralicidic activity-2-like-2 (SKIV2L2). <i>Skiv2l2</i> mRNA was found in the kidney, epididymis and heart as well as the testis. In the testis, <i>Skiv2l2</i> mRNA was shown to be highly expressed in the spermatocytes at stages I to VI. On the other hand, SKIV2L2 protein was found to be predominantly localized in the nuclei of round spermatids. In accordance with the fact that SKIV2L2 belongs to the Ski2 family within the superfamily 2 of RNA helicases, it has been shown that SKIV2L2 has both the RNA-binding and ATPase activities.<br>

Journal

  • Journal of Reproduction and Development

    Journal of Reproduction and Development 57(4), 457-467, 2011-08-01

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

References:  31

Codes

  • NII Article ID (NAID)
    10029593054
  • NII NACSIS-CAT ID (NCID)
    AA10936678
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09168818
  • NDL Article ID
    11216967
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z54-H305
  • Data Source
    CJP  NDL  J-STAGE 
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