FOXO3 Knockdown Accelerates Development of Bovine Primordial Follicles

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Author(s)

Abstract

The objective of the present study was to elucidate the involvement of FOXO3 in the activation of bovine primordial follicles. In immunohistochemistry, FOXO3 was detected in all of the oocytes in primordial and primary follicles. The FOXO3 decreased after treatment with <i>FOXO3</i> small interfering RNAs (siRNAs). Ovarian tissues containing dominantly primordial follicles were treated with <i>FOXO3</i> siRNAs and then xenografted to severe combined immune deficiency (SCID) mice. Two months after xenografting, some primordial follicles developed to the secondary and tertiary stages, and the total percentage of these developing follicles (secondary and tertiary follicles: 18 ± 7%) was higher than in the control grafts treated with control siRNA (7 ± 1%). It is thought that bovine primordial follicle activation is regulated by the FOXO3-dependent mechanism and that knockdown of FOXO3 induces the release of primordial follicles from FOXO3 suppression, initiating their growth. <br>

Journal

  • Journal of Reproduction and Development

    Journal of Reproduction and Development 57(4), 475-480, 2011-08-01

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

References:  24

Cited by:  1

Codes

  • NII Article ID (NAID)
    10029593128
  • NII NACSIS-CAT ID (NCID)
    AA10936678
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09168818
  • NDL Article ID
    11216988
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z54-H305
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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