Characterization and Radio-resistant Function of Manganese Superoxide Dismutase of Rubrobacter radiotolerans
-
- TERATO Hiroaki
- Analytical Research Center for Experimental Sciences, Saga University
-
- SUZUKI Katsuyuki
- Faculty of Engineering, Kinki University
-
- NISHIOKA Nobuhiro
- Faculty of Engineering, Kinki University
-
- OKAMOTO Atsushi
- Faculty of Engineering, Kinki University
-
- SHIMAZAKI-TOKUYAMA Yuka
- Analytical Research Center for Experimental Sciences, Saga University
-
- INOUE Yuko
- Analytical Research Center for Experimental Sciences, Saga University
-
- SAITO Takeshi
- Research Reactor Institute, Kyoto University
この論文をさがす
抄録
Rubrobacter radiotolerans is the most radio-resistant eubacterium without spore-formation in the life cycle, and its D37 is 16,000 Gy against gamma-rays. To understand the molecular mechanism of the high radio-resistance, we purified and characterized superoxide dismutase (SOD) of this organism as enzymatic radical scavenger, and then analyzed its genetic information. The purified SOD protein formed homo-tetramerization of 24,000 Da-monomer, while maintaining its enzymatic activity against potassium cyanide and hydrogen peroxide. We obtained a partial amino acid sequence of the protein and cloned the gene from it. Sequence analysis of the cloned gene indicated that the protein showed a similarity to other bacterial manganese SODs (Mn-SODs). Sequencing for adjacent regions of the gene showed that the gene had promoter elements with an open reading frame for putative PAS/PAC sensor protein at the 5'-adjacent region. Introduction of the gene into Escherichia coli cells lacking intrinsic SOD genes restored the cellular enzymatic activity and resistance to methyl viologen, indicating the gene at work. A mutant cell harboring this gene also became resistant against gamma-rays. The present results suggest that the protein in question is the Mn-SOD of R. radiotolerans, a good candidate as a radio-protection factor for this bacterial radio-resistance.
収録刊行物
-
- Journal of Radiation Research
-
Journal of Radiation Research 52 (6), 735-742, 2011
Journal of Radiation Research 編集委員会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001205216096000
-
- NII論文ID
- 10029652943
-
- NII書誌ID
- AA00705792
-
- ISSN
- 13499157
- 04493060
-
- NDL書誌ID
- 023320639
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可