Nitrate Removal Efficiency and Bacterial Community Dynamics in Denitrification Processes Using Poly (<sc>L</sc>-lactic acid) as the Solid Substrate
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- Takahashi Masaaki
- Department of Environmental and Life Sciences, Toyohashi University of Technology
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- Yamada Takeshi
- Department of Environmental and Life Sciences, Toyohashi University of Technology
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- Tanno Motohiro
- Department of Environmental and Life Sciences, Toyohashi University of Technology
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- Tsuji Hideto
- Department of Environmental and Life Sciences, Toyohashi University of Technology
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- Hiraishi Akira
- Department of Environmental and Life Sciences, Toyohashi University of Technology
Bibliographic Information
- Other Title
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- Nitrate removal efficiency and bacterial community dynamics in denitrification processes using poly (L-lactic acid) as the solid substrate
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Abstract
Laboratory-scale solid-phase denitrification (SPD) reactors for nitrate removal were constructed by acclimating activated sludge with poly (<sc>L</sc>-lactic acid) (PLLA) having weight-average molecular weights (Mw) of 9,900, 12,000, and 45,100 g mol-1. A good nitrate removal rate (3.5-5.3 mg NO3--N g [dry wt]-1 h-1) was found in the reactor containing PLLA of 9,900 g mol-1, whereas the other two reactors with the higher Mw PLLA showed low nitrate removal efficiency. Microbial community dynamics in the low Mw PLLA-acclimated reactor were studied by 16S rRNA gene-targeted PCR-denaturing gradient gel electrophoresis and quinone profiling. Nonmetric multidimensional scaling analyses of these data sets revealed a marked population shift during acclimation of the SPD reactor with low Mw PLLA. The 16S rRNA gene clone library and culture-dependent analyses showed that bacteria belonging to the family Comamonadaceae predominated and played the primary role in denitrification in the PLLA-using reactor; however, none of the bacterial isolates from the reactor degraded PLLA. These results suggest that the nitrate removal property of the PLLA-using SPD reactor is attained through the bioavailability of hydrolysates released abiotically from the solid substrate.<br>
Journal
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- Microbes and Environments
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Microbes and Environments 26 (3), 212-219, 2011
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
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Keywords
Details 詳細情報について
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- CRID
- 1390001204346240128
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- NII Article ID
- 10029653494
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- NII Book ID
- AA11551577
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- COI
- 1:STN:280:DC%2BC3MjpvVahtA%3D%3D
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- ISSN
- 13474405
- 13426311
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- NDL BIB ID
- 11225400
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- PubMed
- 21558675
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed