p-Nitrophenyl β-glycosides of β-1,4-gluco/xylo-disaccharides for the characterization of subsites in endo-xylanases
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- Nishimoto Mamoru
- Enzyme laboratory, National Food Research Institute, National Agriculture and Food Research Organization
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- Kobayashi Atsushi
- Enzyme laboratory, National Food Research Institute, National Agriculture and Food Research Organization
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- Honda Yuji
- Enzyme laboratory, National Food Research Institute, National Agriculture and Food Research Organization
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- Kitaoka Motomitsu
- Enzyme laboratory, National Food Research Institute, National Agriculture and Food Research Organization
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- Hayashi Kiyoshi
- Enzyme laboratory, National Food Research Institute, National Agriculture and Food Research Organization
書誌事項
- タイトル別名
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- p-Nitrophenyl .BETA.-Glycosides of .BETA.-1,4-Gluco/xylo-disaccharides for the Characterization of Subsites in Endo-xylanases
- p Nitrophenyl v glycosides of v 1 4 gluco xylo disaccharides for the characterization of subsites in endo xylanases
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抄録
To investigate the substrate recognition at the minus subsites of glycoside hydrolase family 10 xylanases, the kinetic parameters of four xylanases on all four p-nitrophenyl β-glycosides of β-1,4-gluco/xylo-disaccharides were determined. All four xylanases hydrolyzed all the four substrates examined. The Km values of all the enzymes on the four substrates lined up in the same order, indicating that both the subsites -1 and -2 of all the enzymes prefer xylose to glucose. The comparison of the parameters on the substrates gave detailed information on the substrate recognition at each subsite -1 and -2.
収録刊行物
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 58 (3), 115-118, 2011
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390001206294141056
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- NII論文ID
- 10029656789
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- NII書誌ID
- AA11809133
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- ISSN
- 18807291
- 13447882
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- NDL書誌ID
- 11229893
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可