Cultivation under salt stress increases the concentration of recombinant miraculin in transgenic tomato fruit, resulting in an increase in purification efficiency

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  • Hirai Tadayoshi
    Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Duhita Narendra
    Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Hiwasa-Tanase Kyoko
    Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Ezura Hiroshi
    Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba

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Abstract

Abstract High target protein concentrations in source materials are important for achieving high purification efficiency. We sought to produce the taste-modifying protein, miraculin, in transgenic tomato fruits and then to extract and purify this protein. In order to improve the efficiency of recombinant miraculin purification from transgenic tomatoes, we tested a salt-stress cultivation technique aimed at increasing miraculin concentration in the tomatoes. Two lines of transgenic tomatoes, 56B and 5B, were grown under salt-stress conditions. There was a higher miraculin content per gram of fresh weight in transgenic tomatoes cultivated under salt stress than in tomatoes grown under non-stressed conditions. The observed increase in miraculin concentration was due to an enrichment effect caused by the miniaturization of tomatoes cultivated under salt stress. When the miraculin-enriched tomatoes were used for miraculin purification, the recovery rate of miraculin was higher.

Journal

  • Plant Biotechnology

    Plant Biotechnology 28 (4), 387-392, 2011

    Japanese Society for Plant Biotechnology

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