Effects of Peanut-Skin Procyanidin A1 on Degranulation of RBL-2H3 Cells

• TOMOCHIKA Keisuke

  Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture

• SHIMIZU-IBUKA Akiko

  Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture

• TAMURA Tomoko

  Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture

• MURA Kiyoshi

  Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture

• ABE Naoki

  Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture

• ONOSE Jun-ichi

  Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture

• ARAI Soichi

  Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture

Abstract

Peanut skin contains large amounts of polyphenols having antiallergic effects. We found that a peanut-skin extract (PSE) inhibits the degranulation induced by antigen stimulation of rat basophilic leukemia (RBL-2H3) cells. A low-molecular-weight fraction from PSE, PSEL, also had inhibitory activity against allergic degranulation. A main polyphenol in PSEL was purified by gel chromatography and fractionated by YMC-gel ODS-AQ 120S50 column. Electrospray ionization mass spectrometry (ESI-MS) analysis of the purified polyphenol gave m⁄z 599 [M+Na]⁺. Based on the results of ¹H-NMR, ¹³C-NMR spectra, and optical rotation analysis, the polyphenol was identified as procyanidin A1. It inhibited the degranulation caused by antigen stimulation at the IC₅₀ of 20.3 μM. Phorbol-12-myristate-13-acetate (PMA) and 2,5,-di(tert-butyl)-1,4-hydroquinone (DTBHQ)-induced processes of degranulation were also inhibited by procyanidin A1. These results indicate that peanut-skin procyanidin A1 inhibits degranulation downstream of protein kinase C activation or Ca²⁺ influx from an internal store in RBL-2H3 cells.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 75 (9), 1644-1648, 2011

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• peanut skin
• polyphenol
• procyanidin
• rat basophilic leukemia (RBL)-2H3
• degranulation

Details

• CRID: 1390282681456531584
• NII Article ID: 10029756695
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.110085
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 11252319
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11252319
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110085
• Web Site: https://search.jamas.or.jp/link/ui/2012048104
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed