Expression Analysis of the<i>VTC2</i>and<i>VTC5</i>Genes Encoding GDP-<scp>L</scp>-Galactose Phosphorylase, an Enzyme Involved in Ascorbate Biosynthesis, in<i>Arabidopsis thaliana</i>

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  • GAO Yongshun
    Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
  • BADEJO Adebanjo Ayobamidele
    Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
  • SHIBATA Hitoshi
    Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
  • SAWA Yoshihiro
    Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
  • MARUTA Takanori
    Department of Advanced Bioscience, Faculty of Agriculture, Kinki University
  • SHIGEOKA Shigeru
    Department of Advanced Bioscience, Faculty of Agriculture, Kinki University
  • PAGE Mike
    Biosciences, College of Life and Environmental Sciences, University of Exeter
  • SMIRNOFF Nicholas
    Biosciences, College of Life and Environmental Sciences, University of Exeter
  • ISHIKAWA Takahiro
    Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University

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  • Expression Analysis of the VTC2 and VTC5 Genes Encoding GDP-L-Galactose Phosphorylase, an Enzyme Involved in Ascorbate Biosynthesis, in Arabidopsis thaliana

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Arabidopsis thaliana contains two GDP-L-galactose phosphorylase genes, VTC2 and VTC5, which are critical for ascorbate (AsA) biosynthesis. We investigated the expression levels of both VTC2 and VTC5 genes in wild-type A. thaliana and the AsA deficient mutants during early seedling growth. Ascorbate accumulated to an equal extent in all genotypes up to 5 d post-germination (DPG). The transcript level of VTC2 was dominant, and increased in parallel with AsA accumulation in the wild type. On the other hand, the expression of VTC5 compensated for the reduced VTC2 transcription levels in the AsA deficient mutant vtc2-1 in young seedlings. A luciferase activity assay indicated that the VTC5 promoter was more active in young (2 DPG) cotyledons and that the VTC2 and VTC5 promoters drove a day-to-night variation in expression. The present work provides clues to the precise roles of VTC2 and VTC5 in AsA biosynthesis in A. thaliana at the young seedling stage.

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