Distribution of Bone Marrow-Derived Cells in the Fracture Callus during Plate Fixation in a Green Fluorescent Protein-Chimeric Mouse Model

• UENO Masaki

  Kitasato University School of Medicine, Department of Orthopaedic Surgery

• UCHIDA Kentaro

  Kitasato University School of Medicine, Department of Orthopaedic Surgery

• TAKASO Masashi

  Kitasato University School of Medicine, Department of Orthopaedic Surgery

• MINEHARA Hiroaki

  Kitasato University School of Medicine, Department of Orthopaedic Surgery

• SUTO Kaori

  Kitasato University School of Medicine, Department of Orthopaedic Surgery

• TAKAHIRA Naonobu

  Kitasato University School of Medicine, Department of Orthopaedic Surgery

• STECK Roland

  Institute of Health and Biomedical Innovation, Queensland University of Technology

• SCHUETZ Michael A.

  Institute of Health and Biomedical Innovation, Queensland University of Technology Trauma Services, The Princess Alexandra Hospital

• ITOMAN Moritoshi

  Department of Orthopaedic Surgery, Kyushu Rosai Hospital

抄録

To clarify the distribution of bone-marrow-derived cells in fractures treated by plate fixation, fracture models were created using the green fluorescent protein (GFP) chimeric mouse. We observed 2 types of fracture healing processes with different types of callus formation and cellular events by using Mouse Fix™, a device allowing plate fixation on the mouse femur, and differences in the distribution of bone-marrow-derived cells between the 2 types. The GFP chimeric mice were created by bone marrow transplantation. Fractures were created on the left femurs of mice and stabilized with either rigid (Group R) or flexible (Group F) plates to prepare undecalcified fresh-frozen sections. In Group F, a large external callus and a large intramedullary callus were formed mostly by endochondral ossification. The cells that made up the intramedullary callus and callus in the fracture gap were GFP positive, but most cells of the external callus were not. In Group R, bone union was achieved mostly without external callus formation, bone apposition occurred directly in the gap, and a small intramedullary callus was formed. As observed in Group F, this group had GFP-positive cells in the callus within the fracture gap and in the intramedullary calluses. The results of this study provided direct evidence of the distribution of bone-marrow-derived cells in the callus of fractures treated by plate fixation under different stability conditions.<br>

収録刊行物

• Experimental Animals

  Experimental Animals 60 (5), 455-462, 2011

  公益社団法人 日本実験動物学会

キーワード

• bone marrow
• callus formation
• fracture
• green fluorescent protein
• plate

詳細情報

• CRID: 1390001205046678016
• NII論文ID: 10029835335
• NII書誌ID: AA11032321
• DOI: 10.1538/expanim.60.455
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL書誌ID: 11268606
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11268606
• Web Site: http://www.jstage.jst.go.jp/article/expanim/60/5/60_5_455/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012213069
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可