海洋環境から分離した殺藻性糸状細菌のDNA交雑試験およびRFLP解析

  • 岩元 幸三
    The United Graduate School of Agricultural Science, Kagoshima University
  • 吉川 毅
    Laboratory of Microbiology, Faculty of Fisheries, Kagoshima University
  • 坂田 泰造
    Laboratory of Microbiology, Faculty of Fisheries, Kagoshima University

書誌事項

タイトル別名
  • DNA-DNA Hybridization and RFLP Analysis of Algicidal Filamentous Bacteria Isolated from Marine Environments.

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抄録

Algicidal filamentous bacteria were isolated from shrimp aquaculture ponds and coastal seawaters in Kagoshima Bay, Japan by use of double layer agar plates containing Chaetoceros ceratosporum cells. The isolated strains were tentatively identified as the genus Saprospira and composed of 3 subgroups according to their cell morphology, pigmentation and GC%. On the other hand, RFLP analysis of 16S rDNA and DNA-DNA hybridization of total DNA were performed in order to clarify the taxonomic relationships among the isolates, and it became clear that the isolates were distributed into 4 subgroups. The reference strains of Saprospira grandis showed no DNA-DNA hybridization reaction with total DNAs of all isolates. These molecular biological techniques were found to be useful for rapid identification and classification of algicidal filamentous bacteria.

収録刊行物

  • 水産増殖

    水産増殖 48 (1), 123-130, 2000

    日本水産増殖学会

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