S 型ワムシの高密度輸送法

DOI 日本農学文献記事索引 Web Site 参考文献26件
  • 小磯 雅彦
    独立行政法人水産総合研究センター能登島栽培漁業センター
  • 桑田 博
    独立行政法人水産総合研究センター日本海区水産研究所
  • 萩原 篤志
    長崎大学大学院生産科学研究科

書誌事項

タイトル別名
  • High-density Transportation of the S-type Rotifer <I>Brachionus plicatilis</I>
  • Sガタ ワムシ ノ コウミツド ユソウホウ

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抄録

We aimed at developing a technique for practical mass transportation of the rotifer Brachionus plicatilis (S-type), based on the existing method used in transporting L-type rotifer. Among the two S-type strains used in Japanese public hatcheries, Yaeyama strain showed significantly higher tolerance to low temperature (5°C) than Okayama strain, thus, we used Yaeyama strain in this study. For high density transportation of rotifers cultured at 25°C, pretreatment cooling at 15°C, is effective. Rotifers were packaged at 30,000 ind./ml in 300-1,000 ml seawater. Thereafter, the rotifers were refrigerated at 5°C. The recovery rate of rotifers after these treatments was about 70% after 2 days of storage. Refrigerated rotifers were less viable, and at least one day recovery period at 25°C is necessary before the rotifers can be used as starter of mass culture or food for fish larvae. Based on these results, we conducted a high-density rotifer transportation at 5°C, and 3×108 rotifers were transported to other hatcheries in 48 hours using a commercial delivery service. The recovery rates of rotifers at arrival were more than 70%. These results showed that high-density transportation of using Yaeyama S-type rotifer strain is possible by optimizing the processes we established.

収録刊行物

  • 水産増殖

    水産増殖 58 (2), 203-210, 2010

    日本水産増殖学会

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