Characterization of the Nuclear- and Plastid-Encoded secA-Homologous Genes in the Unicellular Red Alga Cyanidioschyzon merolae

• KOYAMA Yosuke

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University

• TAKIMOTO Koji

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University

• KOJIMA Asuka

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University

• ASAI Kei

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University

• MATSUOKA Satoshi

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University

• MITSUI Toshiaki

  Graduate School of Science and Technology, Niigata University

• MATSUMOTO Kouji

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University

• HARA Hiroshi

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University

• OHTA Niji

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University

Bibliographic Information

Other Title

• Characterization of the Nuclear- and Plastid-Encoded<i>secA</i>-Homologous Genes in the Unicellular Red Alga<i>Cyanidioschyzon merolae</i>

Abstract

SecA is an ATP-driven motor for protein translocation in bacteria and plants. Mycobacteria and listeria were recently found to possess two functionally distinct secA genes. In this study, we found that Cyanidioschyzon merolae, a unicellular red alga, possessed two distinct secA-homologous genes; one encoded in the cell nucleus and the other in the plastid genome. We found that the plastid-encoded SecA homolog showed significant ATPase activity at low temperature, and that the ATPase activity of the nuclear-encoded SecA homolog showed significant activity at high temperature. We propose that the two SecA homologs play different roles in protein translocation.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 75 (10), 2073-2078, 2011

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• <I>secA</I>
• <I>Cyanidioschyzon merolae</I>
• ATPase activity
• temperature dependence

Details

• CRID: 1390282681454630784
• NII Article ID: 10029873431
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.110338
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 11286963
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11286963
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110338
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed