Improvement of Porcine Oocytes with Low Developmental Ability after Fusion of Cytoplasmic Fragments Prepared by Serial Centrifugation

Access this Article

Author(s)

Abstract

We have shown in pigs that oocytes denuded of cumulus cells at 24 h of <i>in vitro</i> maturation culture and subsequently matured for a total of 46 h (DO24 oocytes) have lower cytoplasmic maturity than those matured with cumulus cells for 46 h and then denuded (DO46 oocytes). In the present study, DO24 zona-free oocytes were fused with one (1C) or two (2C) cytoplasmic fragments produced by serial centrifugation ("centri-fusion") of DO46 oocytes (DO24+1C and DO24+2C oocytes, respectively). Groups of (1) DO46 (a control), (2) DO24, (3) DO24+1C and (4) DO24+2C oocytes were partheno-activated by an electrical pulse or fertilized <i>in vitro</i> and subsequently cultured for 6 days. In the fused groups, female pronucleus (FPN) formation rates were higher than that in the DO24 group after parthenogenetic activation (PA); however, the blastocyst rates were intermediate between those of the control and DO24 groups. After <i>in vitro</i> fertilization, the male pronucleus (MPN) formation rates in the fused groups were similar to that in the control group and higher than that in the DO24 group; the normal fertilization rate in the DO24+2C group was higher than that in the DO24 group and similar to that in the control group, resulting in significantly higher blastocyst rates in the DO24+2C and control groups than that in the DO24 group. These results suggest that centri-fusion using ooplasm from fully matured DO46 oocytes can offer a potentially novel approach for restoration of cytoplasmic maturity to oocytes with low developmental ability and subsequent improvement of fertilization and developmental competence.<br>

Journal

  • Journal of Reproduction and Development

    Journal of Reproduction and Development 57(5), 620-626, 2011-10-01

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

References:  42

Cited by:  1

Codes

  • NII Article ID (NAID)
    10029878394
  • NII NACSIS-CAT ID (NCID)
    AA10936678
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09168818
  • NDL Article ID
    11291000
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z54-H305
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
Page Top