Direct Assessments of the Antioxidant Effects of the Novel Collagen Peptide on Reactive Oxygen Species Using Electron Spin Resonance Spectroscopy

• Kobayashi Kyo

  Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan

• Maehata Yojiro

  Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan

• Kawamura Yosuke

  Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan

• Kusubata Masashi

  Nippi Research Institute of Biomatrix, Japan

• Hattori Shunji

  Nippi Research Institute of Biomatrix, Japan

• Tanaka Keisuke

  Nippi Research Institute of Biomatrix, Japan

• Miyamoto Chihiro

  Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan

• Yoshino Fumihiko

  Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan

• Yoshida Ayaka

  Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan

• Wada-Takahashi Satoko

  Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan

• Komatsu Tomoko

  Division of Dentistry for Special Patients, Department of Clinical Care Medicine, Kanagawa Dental College, Japan

• Takahashi Shun-Suke

  Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan

• Lee Masaichi-Chang-Il

  Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan

抄録

In the present study, we evaluated the antioxidant effects of a pepsin-treated novel collagen peptide (P-NCP) on reactive oxygen species (ROS) such as hydroxyl radical (HO^•), superoxide anion radical (O₂^•–), and singlet oxygen (¹O₂), and the effects on cell viability after ultraviolet ray (UV) irradiation of human fibroblasts. We confirmed, using electron spin resonance, that P-NCP directly inhibited HO^• and ¹O₂. Furthermore, addition of P-NCP to fibroblasts inhibited cell death induced by UVA (400 – 315 nm) irradiation in a dose-dependent manner. In addition, the antioxidant effect on ¹O₂ was observed in the peptide fractions rich in Gly, Pro, Hyp, Glu, Ala, and Arg. We found that Gly, Hyp, Glu, and Ala directly scavenged ¹O₂. These results indicated that a peptide sequence including Gly, Hyp, Glu, and Ala could play a key role in the antioxidant effects of P-NCP on ¹O₂. It was suggested that P-NCP can inhibit photo-aging related to ROS owing to its antioxidant effects.

収録刊行物

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 116 (1), 97-106, 2011

  公益社団法人 日本薬理学会

キーワード

• singlet oxygen
• hydroxyl radical
• photo-aging
• human fibroblast
• ultraviolet ray A (UVA) irradiation

詳細情報

• CRID: 1390001205178123392
• NII論文ID: 10029894130
• NII書誌ID: AA11806667
• DOI: 10.1254/jphs.10321fp
• ISSN: 13478648
• ISSN: 13478613
• NDL書誌ID: 11083231
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11083231
• Web Site: https://search.jamas.or.jp/link/ui/2012093376
• 本文言語コード: en
• データソース種別:
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