Myosin Light Chain Kinase / Actin Interaction in Phorbol Dibutyrate-Stimulated Smooth Muscle Cells

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  • Thatcher Sean E.
    Department of Pharmacology, Physiology, and Toxicology, The Joan C. Edwards School of Medicine, Marshall University, USA
  • Fultz Mike E.
    Department of Biology, Morehead State University, USA
  • Tanaka Hideyuki
    Department of Health Sciences, Gunma University, Japan
  • Hagiwara Haruo
    Department of Health Sciences, Gunma University, Japan
  • Zhang Hou-Li
    Department of Molecular and Cellular Pharmacology, Gunma University, Graduate School of Medicine, Japan Department of Pharmacology, Dalian Medical University, PR China
  • Zhang Ying
    Department of Molecular and Cellular Pharmacology, Gunma University, Graduate School of Medicine, Japan Department of Pharmacology, Dalian Medical University, PR China
  • Hayakawa Kohichi
    Department of Molecular and Cellular Pharmacology, Gunma University, Graduate School of Medicine, Japan
  • Yoshiyama Shinji
    Department of Molecular and Cellular Pharmacology, Gunma University, Graduate School of Medicine, Japan
  • Nakamura Akio
    Department of Molecular and Cellular Pharmacology, Gunma University, Graduate School of Medicine, Japan
  • Wang Hong Hui
    Department of Molecular and Cellular Pharmacology, Gunma University, Graduate School of Medicine, Japan
  • Katayama Takeshi
    Department of Molecular and Cellular Pharmacology, Gunma University, Graduate School of Medicine, Japan
  • Watanabe Masaru
    Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Japan
  • Lin Yuan
    Department of Pharmacology, Dalian Medical University, PR China
  • Wright Gary L.
    Department of Pharmacology, Physiology, and Toxicology, The Joan C. Edwards School of Medicine, Marshall University, USA
  • Kohama Kazuhiro
    Department of Pharmacology, Physiology, and Toxicology, The Joan C. Edwards School of Medicine, Marshall University, USA Department of Molecular and Cellular Pharmacology, Gunma University, Graduate School of Medicine, Japan Cell Differentiation and Development Center, Marshall University, USA

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Abstract

Previous work has suggested that in addition to its kinase activity, myosin light chain kinase (MLCK) exhibits non-kinase properties within its N-terminus that could influence cytoskeletal organization of smooth muscle cells (A. Nakamura et al. Biochem Biophys Res Commun. 2008;369:135–143). Myosin ATPase activity measurements indicate that the 26 – 41 peptide of MLCK significantly decreases ATPase activity as the concentration of this peptide increases. Sliding velocity of actin-filaments on myosin and stress responses in skinned smooth muscle tissue are also inhibited. Peptide-mediated uptake and the microinjection technique in cells indicate that the peptide was necessary for actin-filament stabilization. Fluorescence resonance energy transfer analysis indicated that in the presence of MLCK, α-actin but not β-actin remodeled during phorbol 12,13-dibutyrate (PDBu)-induced contractions. PDBu also induced podosomes in the cell. When MLCK expression was down-regulated by introduction of RNAi for MLCK by lentivirus vector into the cells, we failed to observe the podosome induction upon PDBu stimulation. Rescue experiments indicate that the non-kinase activity of MLCK plays an important role in maintaining actin stress fibers and in the PDBu-induced reorganization of actin-filaments in smooth muscle cells.

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