Improvement of Detectable Sensitivity for Enzyme Reaction by Scanning Electrochemical Microscopy with Distance Control System for Immunosensing

• HIRANO Yu

  National Institute of Advanced Industrial, Science and Technology (AIST)

• YASUKAWA Tomoyuki

  Graduate School of Material Science, University of Hyogo

• MASE Yoshiaki

  Graduate School of Environmental Studies, Tohoku University

• OYAMATSU Daisuke

  Graduate School of Environmental Studies, Tohoku University

• SHIKU Hitoshi

  Graduate School of Environmental Studies, Tohoku University

• MIZUTANI Fumio

  Graduate School of Material Science, University of Hyogo

• MATSUE Tomokazu

  Graduate School of Environmental Studies, Tohoku University

Abstract

Detectable sensitivity for immobilized enzymes was improved using a constant-distance mode of scanning electrochemical microscopy (SECM). Redox species generated by an enzyme reaction captured via immunorecognition were detected with a microelectrode used as a probe. The probe maintained considerably close to the target surface (50 nm) allowed the enhancement of the current response because of cycling of redox species between the probe and the captured enzymes. The sensitivity of di(n-butyl)phthalate detection with a competitive assay by using constant-distance mode SECM was at least one order of magnitude higher than that of the probe scanned in a constant z-position.

Journal

• Electrochemistry

  Electrochemistry 80 (1), 30-32, 2012

  The Electrochemical Society of Japan

Keywords

• Scanning Electrochemical Microscopy
• Shear Force Feedback
• Electrochemical Immunosensing
• Competitive Reaction

Details

• CRID: 1390282681472700544
• NII Article ID: 10029981363
• NII Book ID: AN00151637
• DOI: 10.5796/electrochemistry.80.30
• ISSN: 21862451
• ISSN: 13443542
• NDL BIB ID: 023389585
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023389585
• Web Site: http://www.jstage.jst.go.jp/article/electrochemistry/80/1/80_1_30/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Allowed