Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests

• KUSDEMIR Mahmut

  Department of Operative Dentistry, Faculty of Dentistry, University of Yeditepe

• GUNAL Solen

  Oral and Dental Health Center, International Acibadem Hospital

• OZER Fusun

  Department of Preventive and Restorative Sciences, University of Pennsylvania School of Dental Medicine

• IMAZATO Satoshi

  Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry

• IZUTANI Naomi

  Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry

• EBISU Shigeyuki

  Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry

• BLATZ Markus B

  Department of Preventive and Restorative Sciences, University of Pennsylvania School of Dental Medicine

Abstract

This study evaluated the cytotoxicity of self-etching primers/adhesives by direct contact and dentin barrier tests. The three two-step self-etching systems Clearfil SE Bond (CSE), Clearfil Protect Bond (CPB), Prime&Bond NT/NRC (PB) and one-step self-etching systems Reactmer Bond (RB), Clearfil Tri-S Bond (CTS), and Adper Prompt L-Pop (AP) were examined. In direct contact tests, L929 cells were cultured in the presence of diluted solutions (50, 20, 10, and 1%) of primer/conditioner of adhesive systems. For dentin barrier tests, each system was applied onto 0.5 or 1.5 mm thick human dentin assembled in a simple pulp chamber device and incubated for 24 h at 37°C to make the diffusive components contact the L929 cells placed at the bottom of the chamber. The cytotoxic effects were assessed by MTT assay. Cell culture without application of any primers/adhesives served as the control for both tests. One-way ANOVA and Tukey HSD tests were used for statistical analyses. The direct contact tests demonstrated that CSE and CPB were less toxic than the other materials at all dilutions. In the dentin barrier tests, toxic effects of materials were reduced with an increase in thickness of intervening dentin. CSE and CPB showed less cytotoxicity than the other adhesives (p<0.05) when applied to 0.5 mm-thick dentin, and CSE was the least toxic in the 1.5 mm-dentin group (p<0.05). Dentin thickness positively affected biocompatibility of the tested bonding systems. Two-step self-etching systems with HEMA-based primers were more biocompatible than other self-etching adhesives.

Journal

• Dental Materials Journal

  Dental Materials Journal 30 (6), 799-805, 2011

  The Japanese Society for Dental Materials and Devices

Keywords

• Cytotoxicity
• Self-etching Adhesives
• MTT Assay

Details

• CRID: 1390001204718064384
• NII Article ID: 10030026285
• NII Book ID: AA10443149
• DOI: 10.4012/dmj.2011-046
• ISSN: 18811361
• ISSN: 02874547
• Web Site: https://www.jstage.jst.go.jp/article/dmj/30/6/30_2011-046/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012301044
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed