A DIGE PROTEOMIC ANALYSIS FOR LOW-INTENSITY EXERCISE-TRAINED RAT SKELETAL MUSCLE

• YAMAGUCHI WATARU

  <i>Health Promotion and Exercise Program, National Institute of Health and Nutrition</i> <i>Graduate School of Sport Science, Waseda University</i>

• FUJIMOTO ERI

  <i>Health Promotion and Exercise Program, National Institute of Health and Nutrition</i> <i>Graduate School of Sport Science, Waseda University</i>

• HIGUCHI MITSURU

  <i>Faculty of Sport Sciences, Waseda University</i>

• TABATA IZUMI

  <i>Health Promotion and Exercise Program, National Institute of Health and Nutrition</i> <i>Faculty of Health and Sport Science, Ritsumeikan University</i>

Bibliographic Information

Other Title

• 低強度・長時間水泳運動トレーニングによりラット骨格筋で発現するタンパク質のプロテオミクス;2D-DIGE解析
• テイキョウド チョウジカン スイエイ ウンドウ トレーニング ニ ヨリ ラット コッカクキン デ ハツゲン スル タンパクシツ ノ プロテオミクス 2D DIGE カイセキ

Abstract

Background: Exercise training induces various adaptations in skeletal muscles. However, the mechanisms remain unclear. Purpose: Therefore, we conducted 2D-DIGE proteomic analysis, which has not yet been used for elucidating adaptations of skeletal muscle after low-intensity exercise training (LIT). Methods: For five days, rats performed LIT, which consisted of two 3-h swimming exercise with45-m rest between the exercise bouts. 2D-DIGE analysis was conducted on epitrochlearis muscles excised eighteen hours after the final training exercise. Results: Proteomic profiling revealed that, out of 681 detected and matched spots, 22 proteins exhibited changed expression by LIT compared with sedentary rats. All proteins were identified by MALDI-TOF/MS. Conclusion: The proteomic 2D-DIGE analysis following LIT identified expressions of skeletal muscle proteins, includingATPsynα, UQCRC1, dihydrolipoamide dehydrogenase, dihydrolipoamide acetyltransferase, that were not previously reported to change their expressions after exercise-training.

Journal

• Japanese Journal of Physical Fitness and Sports Medicine

  Japanese Journal of Physical Fitness and Sports Medicine 60 (5), 511-518, 2011

  The Japanese Society of Physical Fitness and Sports Medicine

Keywords

• swimming
• epitrochlearis
• proteomics
• low-intensity exercise training

Details

• CRID: 1390282679897734528
• NII Article ID: 10030027192
• NII Book ID: AN00137986
• DOI: 10.7600/jspfsm.60.511
• ISSN: 18814751
• ISSN: 0039906X
• NDL BIB ID: 11283256
• Web Site: http://id.ndl.go.jp/bib/11283256
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11283256
• Web Site: https://www.jstage.jst.go.jp/article/jspfsm/60/5/60_5_511/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012083493
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed