Inhibitory effects of clinical reagents having anti-oxidative activity on transforming growth factor-.BETA.1-induced expression of .ALPHA.-smooth muscle actin in human fetal lung fibroblasts
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- Matsuzawa Yasuo
- Department of Internal Medicine, Toho University School of Medicine, Sakura Hospital
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- Kawashima Tatsuo
- Department of Internal Medicine, Toho University School of Medicine, Sakura Hospital
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- Yamazaki Risa
- Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, Chiba University
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- Yamaura Erika
- Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, Chiba University
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- Makiyama Tomohiko
- Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, Chiba University
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- Fujino Hiromichi
- Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, Chiba University
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- Murayama Toshihiko
- Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, Chiba University
Bibliographic Information
- Other Title
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- Inhibitory effects of clinical reagents having anti-oxidative activity on transforming growth factor-β1-induced expression of α-smooth muscle actin in human fetal lung fibroblasts
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Abstract
Excessive production of transforming growth factor-β1 (TGFβ1) plays an important role in lung fibrosis, in which the differentiation of fibroblasts into myofibroblasts is a key process. Increased formation of reactive oxygen species (ROS) induced by TGFβ1 is a common pathological feature of fibrosis. In the present study, probucol and lovastatin, which are therapeutics used for hyperlipidemia and proposed to act as anti-oxidants, were examined in terms of their effect on TGFβ1-induced formation of ROS and expression of α-smooth muscle actin (αSMA), a myofibroblast marker, in human fetal lung fibroblasts (HFL1 cells). The effects of anti-oxidative enzymes and reagents including N-acetyl-L-cysteine, α-tocopherol, and lecithinized-superoxide dismutase (SOD) on TGFβ1-induced expression of αSMA were also examined. Treatment with probucol (30 µM) and lovastatin (1 µM and 3 µM), in addition to lecithinized-SOD, significantly inhibited the TGFβ1-induced formation of ROS and αSMA. Other anti-oxidants including N-acetyl-L-cysteine had marginal effects on αSMA expression under the conditions. Probucol and lovastatin, established therapeutics, may be worth trying in patients with both lung fibrosis and hypercholesterolemia.
Journal
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- The Journal of Toxicological Sciences
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The Journal of Toxicological Sciences 36 (6), 733-740, 2011
The Japanese Society of Toxicology
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Details 詳細情報について
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- CRID
- 1390282679879286528
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- NII Article ID
- 10030036736
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- NII Book ID
- AN00002808
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- ISSN
- 18803989
- 03881350
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- NDL BIB ID
- 023358479
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed