Application of PCR-Denaturing-Gradient Gel Electrophoresis (DGGE) Method to Examine Microbial Community Structure in Asparagus Fields with Growth Inhibition due to Continuous Cropping

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Growth inhibition due to continuous cropping of asparagus is a major problem; the yield of asparagus in replanted fields is low compared to that in new fields, and missing plants occur among young seedlings. Although soil-borne disease and allelochemicals are considered to be involved in this effect, this is still controversial. We aimed to develop a technique for the biological field diagnosis of growth inhibition due to continuous cropping. Therefore, in this study, fungal community structure and <i>Fusarium</i> community structure in continuously cropped fields of asparagus were analyzed by polymerase chain reaction/denaturing-gradient gel electrophoresis (PCR-DGGE). Soil samples were collected from the Aizu region of Fukushima Prefecture, Japan. Soil samples were taken from both continuously cropped fields of asparagus with growth inhibition and healthy neighboring fields of asparagus. The soil samples were collected from the fields of 5 sets in 2008 and 4 sets in 2009. We were able to distinguish between pathogenic and non-pathogenic <i>Fusarium</i> by using Alfie1 and Alfie2GC as the second PCR primers and PCR-DGGE. Fungal community structure was not greatly involved in the growth inhibition of asparagus due to continuous cropping. By contrast, the band ratios of <i>Fusarium oxysporum</i> f. sp. <i>asparagi</i> in growth-inhibited fields were higher than those in neighboring healthy fields. In addition, there was a positive correlation between the band ratios of <i>Fusarium oxysporum</i> f. sp. <i>asparagi</i> and the ratios of missing asparagus plants. We showed the potential of biological field diagnosis of growth inhibition due to continuous cropping of asparagus using PCR-DGGE.<br>

収録刊行物

  • Microbes and environments

    Microbes and environments 27(1), 43-48, 2012-03-01

    日本微生物生態学会・日本土壌微生物学会

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各種コード

  • NII論文ID(NAID)
    10030122702
  • NII書誌ID(NCID)
    AA11173196
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13426311
  • NDL 記事登録ID
    023470105
  • NDL 請求記号
    Z54-J644
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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