Isolation of Two Novel Marine Ethylene-Assimilating Bacteria, <i>Haliea</i> Species ETY-M and ETY-NAG, Containing Particulate Methane Monooxygenase-like Genes

• Suzuki Toshihiro

  Graduate School of Regional Environment Systems, Shibaura Institute of Technology

• Nakamura Takamichi

  Chugai Technos Corp.

• Fuse Hiroyuki

  College of Systems Engineering and Science, Shibaura Institute of Technology

Bibliographic Information

Other Title

• Isolation of Two Novel Marine Ethylene-Assimilating Bacteria, Haliea Species ETY-M and ETY-NAG, Containing Particulate Methane Monooxygenase-like Genes

Abstract

Two novel ethylene-assimilating bacteria, strains ETY-M and ETY-NAG, were isolated from seawater around Japan. The characteristics of both strains were investigated, and phylogenetic analyses of their 16S rRNA gene sequences showed that they belonged to the genus Haliea. In C1–4 gaseous hydrocarbons, both strains grew only on ethylene, but degraded ethane, propylene, and propane in addition to ethylene. Methane, n-butane, and i-butane were not utilized or degraded by either strain. Soluble methane monooxygenase-type genes, which are ubiquitous in alkene-assimilating bacteria for initial oxidation of alkenes, were not detected in these strains, although genes similar to particulate methane monooxygenases (pMMO)/ammonia monooxygenases (AMO) were observed. The phylogenetic tree of the deduced amino acid sequences formed a new clade near the monooxygenases of ethane-assimilating bacteria similar to other clades of pMMOs in type I, type II, and Verrucomicrobia methanotrophs and AMOs in alpha and beta proteobacteria.<br>

Journal

• Microbes and Environments

  Microbes and Environments 27 (1), 54-60, 2012

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• short-chain alkene
• <i>Haliea</i>
• particulate methane monooxygenase (<i>pmo</i>)

Details

• CRID: 1390001204344905984
• NII Article ID: 10030122761
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.me11256
• COI: 1:STN:280:DC%2BC38vitFSksA%3D%3D
• ISSN: 13474405
• ISSN: 13426311
• NDL BIB ID: 023470122
• PubMed: 22307463
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023470122
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/27/1/27_ME11256/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed