Vertical Profiles of Abundance and Potential Activity of Methane-Oxidizing Bacteria in Sediment of Lake Biwa, Japan

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Vertical profiles of the abundance, community composition, and potential activity of methane-oxidizing bacteria (MOB) were investigated in the sediment of Lake Biwa. Sediment samples were obtained from two sites at different water depths. The abundance of MOB was assessed as the copy number of the <i>pmoA</i> gene (encoding the alpha subunit of particulate methane monooxygenase), measured with quantitative real-time PCR. Abundance of the <i>pmoA</i> gene peaked in the 5-8 cm layer of the sediment from both sites. MOB community composition was investigated by denaturing gradient gel electrophoresis (DGGE) analysis of <i>pmoA</i> and 16S rRNA genes. The band patterns observed in DGGE did not significantly differ with sediment depths or sampling sites. Sequence analysis of the DGGE bands indicated the dominance of the genus <i>Methylobacter</i>. Potential activity, which was measured in the presence of sufficient amounts of methane and oxygen, decreased linearly from the sediment surface to deeper layers. These results suggest that the <i>pmoA</i> gene copy number cannot be regarded as an indicator of aerobic MOB that retain potential activity in sediments.<br>

収録刊行物

  • Microbes and environments

    Microbes and environments 27(1), 67-71, 2012-03-01

    日本微生物生態学会・日本土壌微生物学会

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各種コード

  • NII論文ID(NAID)
    10030122839
  • NII書誌ID(NCID)
    AA11173196
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13426311
  • NDL 記事登録ID
    023470138
  • NDL 請求記号
    Z54-J644
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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