Trichloroethylene enhances TCR-CD3-induced proliferation of CD8⁺ rather than CD4⁺ T cells Trichloroethylene enhances TCR-CD3-induced proliferation of CD8^+ rather than CD4^+ T cells

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Trichloroethylene (TCE) is suspected as a potent immunomodulator that accelerates the development of allergic diseases. We previously reported that TCE promotes ovalbumin (OVA)-induced active cutaneous anaphylaxis, including enhancing antigen-specific serum IgE levels and splenic lymphocyte proliferation. However, the target cells and molecular mechanism through which TCE modulates antigen-specific immune responses remain unclear. To identify a potential underlying mechanism, we investigated whether TCE modulates T cell receptor (TCR)-induced T cell activation and proliferation <i>in vitro</i>. TCE enhanced T cell proliferation primed by anti-CD3 antibody, but not concanavalin A, in a dose-dependent fashion. In addition, TCE enhanced anti-CD3-primed proliferation of CD8<sup>+</sup> rather than CD4<sup>+</sup> T cells. Consistent with this result, TCE markedly enhanced the Lck phosphorylation mediated by anti-CD3 antibody in CD8<sup>+</sup> but not CD4<sup>+</sup> T cells. Furthermore, we analyzed the effect of TCE exposure via drinking water for 2 weeks on splenocyte populations in non-immunized and OVA-immunized mice. In OVA-immunized mice, TCE (3 mg/l) significantly expanded CD3<sup>+</sup>, CD8<sup>+</sup> and CD4<sup>+</sup> cell populations, however the effect at the lower concentration was significant only in the CD8<sup>+</sup> populations, whereas TCE had no effect on these cells population in non-immunized mice. These findings suggest that TCE enhances TCR-CD3-induced proliferation of CD8<sup>+</sup> rather than CD4<sup>+</sup> cells and disrupts various activities of peripheral T cells.

収録刊行物

  • Journal of toxicological sciences

    Journal of toxicological sciences 37(2), 381-387, 2012-04-01

    一般社団法人 日本毒性学会

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各種コード

  • NII論文ID(NAID)
    10030126895
  • NII書誌ID(NCID)
    AN00002808
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    03881350
  • NDL 記事登録ID
    023674701
  • NDL 請求記号
    Z19-1022
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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