Glioma Immunotherapy With Combined Autologous Tumor Cell and Endothelial Cell Vaccine In Vivo

• SAKAMOTO Noriaki

  Department of Neurosurgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba

• UEMAE Youji

  Department of Neurosurgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba

• ISHIKAWA Eiichi

  Department of Neurosurgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba

• TAKANO Shingo

  Department of Neurosurgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba

• NAKAI Kei

  Department of Neurosurgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba

• YAMAMOTO Tetsuya

  Department of Neurosurgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba

• ZABORONOK Alexander

  Department of Neurosurgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba

• MATSUMURA Akira

  Department of Neurosurgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba

Abstract

Combined vaccines containing GL261 murine glioma cells and F-2 murine endothelial cells fixed with glutaraldehyde-phosphate buffered saline were injected into the intradermal tissue of the tail base of C57BL/6 mice. After the vaccination, GL261 cells were injected subcutaneously into the left flank of the mice. Vaccination with fixed F-2 cells induced the development of relatively high amounts of interferon-gamma-releasing cells after in vitro re-stimulation with vascular endothelial growth factor-receptor 2 peptide. Tumor growth was inhibited after preventive use of the combined vaccine, prepared from GL261 and F-2 cells. Tumor specimens obtained from the combined vaccine group in a therapeutic experiment showed significantly decreased vessel count. Glioma immunotherapy with a combined vaccine prepared from tumor cells and endothelial cells might represent a new clinical strategy, as such combinations may theoretically affect both high-grade glioma cells and their environment.<br>

Journal

• Neurologia medico-chirurgica

  Neurologia medico-chirurgica 52 (4), 194-201, 2012

  The Japan Neurosurgical Society

Keywords

• endothelial vaccine
• immunotherapy
• glioma
• human umbilical vein endothelial cell
• endothelial cell

Details

• CRID: 1390282680034624768
• NII Article ID: 10030127755
• NII Book ID: AN00358613
• DOI: 10.2176/nmc.52.194
• COI: 1:STN:280:DC%2BC38rmvFWguw%3D%3D
• ISSN: 13498029
• ISSN: 04708105
• PubMed: 22522329
• Web Site: https://www.jstage.jst.go.jp/article/nmc/52/4/52_4_194/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2013155941
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed