Effects of a calcium phosphate cement on mineralized nodule formation compared with endodontic cements

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The aim of this study was to investigate mineralizing ability of a premixed calcium phosphate cement (premixed-CPC) compared to mineral trioxide aggregate (MTA) and zinc oxide eugenol cement (SuperEBA) in ROS17/2.8 cells. The measurements of cell proliferation, alkaline phosphatase (ALPase) activity and mineralized nodule formation in the presence or absence (control) of the test materials were performed using a cell culture insert method with the test materials placed on a porous membrane of culture plate insert. Mineralized nodules were detected by staining with alizarin red, and the calcium content of the mineralized nodules was determined quantitatively using a calcium assay kit. Premixed-CPC and MTA indicated significantly higher cell proliferation, ALPase activity, mineralized nodule formation, and calcium content in nodules than those of SuperEBA (<I>p</I><0.05). The present results suggest that premixed-CPC has the same mineralizing ability as MTA.

収録刊行物

  • Dental materials journal

    Dental materials journal 31(1), 92-97, 2012-01-01

    日本歯科理工学会

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各種コード

  • NII論文ID(NAID)
    10030128348
  • NII書誌ID(NCID)
    AA10443149
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    02874547
  • データ提供元
    CJP書誌  J-STAGE 
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