Enhanced osteoblast response to electrical discharge machining surface

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  • OTSUKA Fukunaga
    Department of Oral Biomaterials and Technology, Showa University School of Dentistry
  • KATAOKA Yu
    Department of Oral Pathology, Showa University School of Dentistry
  • MIYAZAKI Takashi
    Department of Oral Biomaterials and Technology, Showa University School of Dentistry

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Abstract

The purpose of this study is to investigate the surface characteristics and biocompatibility of titanium (Ti) surfaces modified by wire electrical discharge machining (EDM). EDM surface characteristics were evaluated by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), thin-film X-ray diffractometry (XRD) and contact angle measurements. MC3T3-E1 cell morphology, attachment and proliferation, as well as analysis of osteoblastic gene expressions, on machined surfaces and EDM surfaces were also evaluated. EDM surfaces exhibited high super hydrophilicity, due to high surface energy. XPS and XRD revealed that a passive oxide layer with certain developing thickness onto. EDM surfaces promoted cell attachment, but restrained proliferation. Counted cell numbers increased significantly on the machined surfaces as compared to the EDM surfaces. Real-time PCR analyses showed significantly higher relative mRNA expression levels of osteoblastic genes (ALP, osteocalcin, Runx2, Osterix) in cells cultured on the EDM surfaces as compared to cells cultured on the machined surfaces.

Journal

  • Dental Materials Journal

    Dental Materials Journal 31 (2), 309-315, 2012

    The Japanese Society for Dental Materials and Devices

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