Effects of Autologous Bone Marrow Mononuclear Cells Implantation in Canine Model of Pulmonary Hypertension

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著者

    • LUAN Yun
    • Central Research Laboratory, The Second Hospital of Shandong University
    • ZHANG Zhao-Hua
    • Department of Pediatrics, The Second Hospital of Shandong University
    • WEI De-E
    • Department of Gynecology and Obstetrics, Jinan Central Hospital Affiliated to Shandong University
    • LU Yan
    • Hospital of Beijing University of Aeronautics and Astronautics
    • WANG Yi-Biao
    • Department of Pediatrics, The Second Hospital of Shandong University

抄録

<b><i>Background:</i></b> We investigated the safety and feasibility of intratracheal administration of autologous bone marrow-derived mononuclear cells (ABM-MNCs) and observed the effects in a canine model of pulmonary hypertension (PH). <b><i>Methods and Results:</i></b> The PH model was induced by intravenous injection of 3mg/kg dehydromonocrotaline (DMCT) via the right atrium. Two weeks after DMCT administration, the animals received 4 different treatments (n=10 in each group): (I) negative control group; (II): ABM-MNCs group; (III) PH group; (IV) PH+ABM-MNCs group. Six weeks after injection of cells (10<sup>7</sup>), the hemodynamic data were significantly improved in group IV compared with group III (P<0.05). The ratio of right ventricular weight to left ventricular plus septal weight was significantly decreased in group IV compared with group III (P<0.05). The mRNA levels of vascular endothelial growth factor, preproendothelin-1, interleukin-6 and tumor necrosis factor-α were significantly improved in group IV compared with group III (P<0.05). The immunofluorescence result showed that 6 weeks after administration ABM-MNCs could differentiate into pulmonary vascular endothelial cells. <b><i>Conclusions:</i></b> Six weeks after intratracheal administration, ABM-MNCs significantly improved the impairment caused by DMCT in a canine model of PH (ie, decreased pulmonary arteriolar narrowing, alveolar septum thickening and right ventricular hypertrophy, enhanced angiogenesis) and this provides a firm foundation for a clinical trial. (<i>Circ J</i> 2012; <b>76:</b> 977-985)<br>

収録刊行物

  • Circulation journal : official journal of the Japanese Circulation Society

    Circulation journal : official journal of the Japanese Circulation Society 76(4), 977-985, 2012-03-25

    一般社団法人 日本循環器学会

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各種コード

  • NII論文ID(NAID)
    10030131586
  • NII書誌ID(NCID)
    AA11591968
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13469843
  • データ提供元
    CJP書誌  J-STAGE 
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