Hypoxic Preconditioning Enhances Angiogenic Potential of Bone Marrow Cells With Aging-Related Functional Impairment

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著者

    • KUBO Masayuki
    • Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine
    • LI Tao-Sheng
    • Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine
    • KURAZUMI Hiroshi
    • Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine
    • TAKEMOTO Yoshihiro
    • Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine
    • OHSHIMA Mako
    • Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine
    • KATSURA Shunsaku
    • Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine
    • MORIKAGE Noriyasu
    • Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine
    • FURUTANI Akira
    • Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine
    • HAMANO Kimikazu
    • Department of Surgery and Clinical Science, Yanaguchi University Graduate School of Medicine

抄録

Background: Hypoxic preconditioning of bone marrow cells (BMCs) from young healthy individuals can enhance the cells' therapeutic potential. Considering that the response to hypoxia may differ according to the quality of the cells, we assessed the effect of hypoxic preconditioning on BMCs from aged mice and compared the difference in response between BMCs from aged and young mice. Methods and Results: BMCs from young (3 months) and aged (20-22 months) mice were subjected to hypoxic preconditioning by culture for 24 h in 2% O2. Compared with BMCs from young mice, those from aged mice showed significantly fewer CD34- or c-kit-positive stem cells, higher expression of p53, and lower telomerase activity. Adhesion, survival and angiogenic potency were also lower in BMCs from aged mice, indicating an aging-related impairment. Hypoxia-preconditioned BMCs from aged mice showed enhanced adhesion, survival, and angiogenic potency with the in vitro assessments, as well as the in vivo implantation into ischemic hindlimbs. All the enhancements by hypoxic preconditioning were comparable between BMCs from aged and young mice, although the angiogenic potential of BMCs with and without hypoxic preconditioning was lower in old mice compared with young mice. Conclusions: Similar responses to hypoxia by BMCs from both aged and young mice suggest that hypoxic preconditioning could be a useful method of enhancing the angiogenic potential of BMCs.

<b><i>Background:</i></b> Hypoxic preconditioning of bone marrow cells (BMCs) from young healthy individuals can enhance the cells' therapeutic potential. Considering that the response to hypoxia may differ according to the quality of the cells, we assessed the effect of hypoxic preconditioning on BMCs from aged mice and compared the difference in response between BMCs from aged and young mice. <b><i>Methods and Results:</i></b> BMCs from young (3 months) and aged (20-22 months) mice were subjected to hypoxic preconditioning by culture for 24h in 2% O<sub>2</sub>. Compared with BMCs from young mice, those from aged mice showed significantly fewer CD34- or c-kit-positive stem cells, higher expression of p53, and lower telomerase activity. Adhesion, survival and angiogenic potency were also lower in BMCs from aged mice, indicating an aging-related impairment. Hypoxia-preconditioned BMCs from aged mice showed enhanced adhesion, survival, and angiogenic potency with the in vitro assessments, as well as the in vivo implantation into ischemic hindlimbs. All the enhancements by hypoxic preconditioning were comparable between BMCs from aged and young mice, although the angiogenic potential of BMCs with and without hypoxic preconditioning was lower in old mice compared with young mice. <b><i>Conclusions:</i></b> Similar responses to hypoxia by BMCs from both aged and young mice suggest that hypoxic preconditioning could be a useful method of enhancing the angiogenic potential of BMCs. (<i>Circ J</i> 2012; <b>76:</b> 986-994)<br>

収録刊行物

  • Circulation journal : official journal of the Japanese Circulation Society

    Circulation journal : official journal of the Japanese Circulation Society 76(4), 986-994, 2012-03-25

    日本循環器学会

参考文献:  37件中 1-37件 を表示

被引用文献:  1件中 1-1件 を表示

各種コード

  • NII論文ID(NAID)
    10030131630
  • NII書誌ID(NCID)
    AA11591968
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13469843
  • データ提供元
    CJP書誌  CJP引用  IR  J-STAGE 
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