Novel Device That Produces Carbon Dioxide Mist for Myocardial Infarction Treatment in Rats

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Author(s)

    • YAMAZAKI Takanori
    • Department of Internal Medicine and Cardiology, Osaka City University Medical School
    • NAKAMURA Yasuhiro
    • Department of Internal Medicine and Cardiology, Osaka City University Medical School
    • HANATANI Akihisa
    • Department of Internal Medicine and Cardiology, Osaka City University Medical School
    • SHIMADA Kenei
    • Department of Internal Medicine and Cardiology, Osaka City University Medical School
    • MURO Takashi
    • Department of Internal Medicine and Cardiology, Osaka City University Medical School
    • IWAO Hiroshi
    • Department of Pharmacology, Osaka City University Medical School
    • YOSHIYAMA Minoru
    • Department of Internal Medicine and Cardiology, Osaka City University Medical School

Abstract

<b><i>Background:</i></b> Carbon dioxide (CO<sub>2</sub>) baths have been used to treat a variety of diseases, but developing an artificial bath of highly concentrated CO<sub>2</sub> is difficult. Here, we tried the efficacy of a novel device instead of a CO<sub>2</sub> bath. <b><i>Methods and Results:</i></b> Using a device equipped with double fluid nozzles, CO<sub>2</sub> gas and H<sub>2</sub>O were compounded and compressed at 4 barometric pressures. As a result, CO<sub>2</sub> gas was dissolved in H<sub>2</sub>O, which contained a few micrometers of CO<sub>2</sub> particles, namely, a CO<sub>2</sub> mist. Wistar rats with myocardial infarction (MI) by ligation of the left coronary artery were percutaneously administered CO<sub>2</sub> mist or CO<sub>2</sub> gas alone or no treatment for 30min daily. With regard to tissue blood flow during treatment, the group treated with CO<sub>2</sub> mist had significantly increased tissue oxygenated hemoglobin levels and tissue saturation levels, and significantly decreased deoxygenated hemoglobin levels compared with the group treated with CO<sub>2</sub> gas. After 4 weeks treatment, the group treated with CO<sub>2</sub> mist had a significantly improved ejection fraction by echocardiography compared with the untreated group. Interestingly, the group treated with CO<sub>2</sub> mist had significantly increased nitrate concentrations in serum and vascular endothelial growth factor mRNA expression levels in the myocardium compared with the untreated group. <b><i>Conclusions:</i></b> Our new mist production device may be potentially useful for the treatment of heart failure caused by MI. (<i>Circ J</i> 2012; <b>76:</b> 1203-1212)<br>

Journal

  • Circulation Journal

    Circulation Journal 76(5), 1203-1212, 2012-04-25

    The Japanese Circulation Society

References:  25

Cited by:  1

Codes

  • NII Article ID (NAID)
    10030132718
  • NII NACSIS-CAT ID (NCID)
    AA11591968
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13469843
  • Data Source
    CJP  CJPref  J-STAGE 
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