Urotensin II Stimulates Vascular Endothelial Growth Factor Secretion From Adventitial Fibroblasts in Synergy With Angiotensin II

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著者

    • SONG Nana
    • Department of Cardiology, Peking University First Hospital
    • DING Wenhui
    • Department of Cardiology, Peking University First Hospital
    • CHU Songyun
    • Department of Cardiology, Peking University First Hospital
    • ZHAO Jing
    • Department of Cardiology, Peking University First Hospital
    • DONG Xiao
    • Department of Cardiology, Peking University First Hospital
    • DI Beibing
    • Department of Cardiology, Peking University First Hospital
    • TANG Chaoshu
    • Department of Physiology and Pathophysiology, Peking University

抄録

<b><i>Background:</i></b> The adventitia plays an important role in and is considered to be the initiating site for vascular remodeling. Urotensin II (UII) and angiotensin II (Ang II) are the two most important vascular peptides involved in vascular remodeling in the adventitia. Nevertheless, little is known about their effect on the expression of vascular endothelial growth factor (VEGF). It was hypothesized that both UII and Ang II could induce VEGF expression in adventitial fibroblasts and VEGF may play a role in cell proliferation and collagen I synthesis induced by UII or Ang II. <b><i>Methods and Results:</i></b> Growth-arrested adventitial fibroblasts were incubated in serum-free medium with UII and/or Ang II and inhibitors of the mitogen-activated protein kinase (MAPK) pathway or VEGF-neutralizing antibodies. The VEGF expression was evaluated using enzyme-linked immunosorbent assay (ELISA), while the proliferation and collagen I synthesis were detected using methyl thiazol tetrazolium (MTT) assay and ELISA. It was found that: (1) both UII and Ang II could stimulate VEGF expression in adventitial fibroblasts and they had a synergistic effect; (2) MAPK pathway inhibitors could inhibit VEGF secretion induced by UII and/or Ang II; and (3) VEGF-neutralizing antibodies could inhibit UII/Ang II-induced cell proliferation and collagen synthesis in adventitial fibroblasts. <b><i>Conclusions:</i></b> Induction of VEGF expression may be a new mechanism involved in vascular remodeling for UII and Ang II. (<i>Circ J</i> 2012; <b>76:</b> 1267-1273)<br>

収録刊行物

  • Circulation journal : official journal of the Japanese Circulation Society

    Circulation journal : official journal of the Japanese Circulation Society 76(5), 1267-1273, 2012-04-25

    一般社団法人 日本循環器学会

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各種コード

  • NII論文ID(NAID)
    10030133017
  • NII書誌ID(NCID)
    AA11591968
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13469843
  • データ提供元
    CJP書誌  J-STAGE 
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