Multicolor Cathodoluminescence Microscopy for Biological Imaging with Nanophosphors

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著者

    • Furukawa Taichi Furukawa Taichi
    • Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan
    • Ashida Masaaki
    • Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan
    • Araki Tsutomu
    • Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan
    • Hashimoto Mamoru
    • Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan

抄録

We report the first demonstration of a multicolor high-spatial-resolution imaging technique for observation of biological cells using cathodoluminescence from nanophosphors. Three kinds of rare-earth-doped nanophosphors were injected into J744A.1 macrophages, and the spatial distribution of nanophosphors was visualized by using a scanning electron microscope cathodoluminescence (SEM-CL) system. The spectral bandwidth of the phosphors was narrow enough to distinguish the types of the phosphors. CL images of the nanophosphors on Si substrates were obtained with high resolution comparable to that of SEM images. These nanophosphors will be candidates to image more than two kinds of biological molecules at high resolution.

収録刊行物

  • Applied physics express

    Applied physics express 4(11), 112402-112402-3, 2011-11-25

    The Japan Society of Applied Physics

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各種コード

  • NII論文ID(NAID)
    10030153369
  • NII書誌ID(NCID)
    AA12295133
  • 本文言語コード
    EN
  • 資料種別
    SHO
  • ISSN
    18820778
  • NDL 記事登録ID
    023321129
  • NDL 請求記号
    Z78-A526
  • データ提供元
    CJP書誌  NDL  JSAP 
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