Real-time Imaging of RNA Expression in Living Cells Using Bispyrene-modified RNA Probes

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著者

    • WAKI Reiko
    • Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology
    • YAMAYOSHI Asako
    • Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology
    • KOBORI Akio
    • Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology
    • MURAKAMI Akira
    • Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology

抄録

We successfully monitored time-dependent RNA expression profiles using bispyrene-modified RNA probe in living cervical carcinoma cells stimulated with EGF. Fluorescence microscopy images clearly showed the RNA expression profile, and our time-dependent profiles were in good agreement with those obtained by RT-PCR. This suggests that our novel method can analyze cellular responses to specific stimuli in a time-dependent manner at a single-cell level.

収録刊行物

  • Chemistry letters

    Chemistry letters 40(11), 1247-1248, 2011-11-05

    公益社団法人 日本化学会

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各種コード

  • NII論文ID(NAID)
    10030220013
  • NII書誌ID(NCID)
    AA00603318
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    03667022
  • データ提供元
    CJP書誌  J-STAGE 
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