Rapid and Convenient Sample Preparation in a Single Tube Using Magnetic Beads for Fluorescence Detection of Single Nucleotide Variation Based on Oligonucleotide Ligation

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著者

    • MORIMOTO Chika
    • Department of Chemical Engineering and Materials Science, Doshisha University
    • HAGIHARA Kenta
    • Department of Chemical Engineering and Materials Science, Doshisha University

抄録

We here describe a rapid and convenient sample preparation in a single tube for detecting single nucleotide variation based on oligonucleotide ligation assay (OLA) using a biotinylated primer and a set of four different fluorophore-labeled primers. Only one of the fluorophore-labeled primers that was fully complementary to a DNA template was ligated with the biotin-primer by DNA ligase. We isolated the ligated fragments that carried a biotin at the 3′-end by capture with streptavidin-coated magnetic beads. The distinct fluorescence signature of each ligation product coded the single nucleotide differences.

収録刊行物

  • Chemistry letters

    Chemistry letters 41(2), 135-137, 2012-02-05

    公益社団法人 日本化学会

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  • electronically on the CSJ-Journal

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各種コード

  • NII論文ID(NAID)
    10030222999
  • NII書誌ID(NCID)
    AA00603318
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    03667022
  • データ提供元
    CJP書誌  J-STAGE 
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