Multiple-animal MR Imaging using a 3T Clinical Scanner and Multi-channel Coil for Volumetric Analysis in a Mouse Tumor Model

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著者

    • MITSUDA Minoru
    • Functional Imaging Division, Research Center for Innovative Oncology, National Cancer Center Hospital East
    • YAMAGUCHI Masayuki
    • Functional Imaging Division, Research Center for Innovative Oncology, National Cancer Center Hospital East
    • FURUTA Toshihiro
    • Functional Imaging Division, Research Center for Innovative Oncology, National Cancer Center Hospital East
    • NIITSU Mamoru
    • Graduate School of Human Health Sciences, Tokyo Metropolitan University
    • FUJII Hirofumi
    • Functional Imaging Division, Research Center for Innovative Oncology, National Cancer Center Hospital East

抄録

Purpose: Multiple small-animal magnetic resonance (MR) imaging to measure tumor volume may increase the throughput of preclinical cancer research assessing tumor response to novel therapies. We used a clinical scanner and multi-channel coil to evaluate the usefulness of this imaging to assess experimental tumor volume in mice.<br> Methods: We performed a phantom study to assess 2-dimensional (2D) geometric distortion using 9-cm spherical and 32-cell (8×4 one-cm<sup>2</sup> grids) phantoms using a 3-tesla clinical MR scanner and dedicated multi-channel coil composed of 16 5-cm circular coils. Employing the multi-channel coil, we simultaneously scanned 6 or 8 mice bearing sarcoma 180 tumors. We estimated tumor volume from the sum of the product of tumor area and slice thickness on 2D spin-echo images (repetition time/echo time, 3500/16 ms; in-plane resolution, 0.195×0.195×1 mm<sup>3</sup>). After MR acquisition, we excised and weighed tumors, calculated reference tumor volumes from actual tumor weight assuming a density of 1.05 g/cm<sup>3</sup>, and assessed the correlation between the estimated and reference volumes using Pearson's test.<br> Results: Two-dimensional geometric distortion was acceptable below 5% in the 9-cm spherical phantom and in every cell in the 32-cell phantom. We scanned up to 8 mice simultaneously using the multi-channel coil and found 11 tumors larger than 0.1 g in 12 mice. Tumor volumes were 1.04±0.73 estimated by MR imaging and 1.04±0.80 cm<sup>3</sup> by reference volume (average±standard deviation) and highly correlated (correlation coefficient, 0.995; <i>P</i><0.01, Pearson's test).<br> Conclusion: Use of multiple small-animal MR imaging employing a clinical scanner and multi-channel coil enabled accurate assessment of experimental tumor volume in a large number of mice and may facilitate high throughput monitoring of tumor response to therapy in preclinical research.<br>

収録刊行物

  • Magnetic resonance in medical sciences : MRMS

    Magnetic resonance in medical sciences : MRMS 10(4), 229-237, 2011-12-01

    Japanese Society for Magnetic Resonance in Medicine

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各種コード

  • NII論文ID(NAID)
    10030284594
  • NII書誌ID(NCID)
    AA11648770
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13473182
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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