Evaluation of Concanavalin A-induced Acute Liver Injury in Rats using an Empirical Mathematical Model and Dynamic Contrast-enhanced MR Imaging with Gd-EOB-DTPA

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著者

    • MORIYAMA Yuki
    • Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University
    • SAITO Shigeyoshi
    • Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University
    • KOBAYASHI Shuichiro
    • Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University
    • OGIHARA Ryota
    • Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University
    • KOTO Daichi
    • Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University
    • KITAMURA Akihiro
    • Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University
    • MATSUSHITA Taro
    • Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University
    • NISHIURA Motoko
    • Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University
    • MURASE Kenya
    • Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University

抄録

Purpose: We evaluated concanavalin A (Con A)-induced acute hepatic injury in rats using an empirical mathematical model (EMM) and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) with gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA).<br> Materials and Methods: We allocated 18 rats into 3 groups of six each and intravenously injected them with either 10 mg/kg body weight (BW) of Con A (Con A [10] group), 20 mg/kg BW of Con A (Con A [20] group), or a single dose of of saline (4 mL/kg BW, normal control group). We performed the DCE-MRI studies using Gd-EOB-DTPA (0.025 mmol Gd/kg; 0.1 mL/kg BW) as the contrast agent 24 hours after injection of Con A or saline. We then sampled blood, measured serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and calculated the rate of contrast uptake (α), rate of contrast washout (β), area under the curve (<i>AUC</i>), time to maximum relative enhancement (<i>RE</i>) (<i>T</i><sub>max</sub>), and elimination half-life of <i>RE</i> (<i>T</i><sub>1/2</sub>) from the time-signal intensity curves using the EMM.<br> Results: β values were significantly smaller in the Con A (10) and Con A (20) groups than the control group, but α did not differ significantly among the 3 groups. The <i>AUC</i> value was significantly greater in the Con A (10) group than controls, and the <i>T</i><sub>max</sub> and <i>T</i><sub>1/2</sub> values were significantly greater in the Con A (20) group than controls. The β, <i>T</i><sub>max</sub>, and <i>T</i><sub>1/2</sub> values correlated significantly with AST and ALT.<br> Conclusion: In conclusion, the EMM is useful for evaluating Con A-induced acute hepatic injury using DCE-MRI with Gd-EOB-DTPA.<br>

収録刊行物

  • Magnetic resonance in medical sciences : MRMS

    Magnetic resonance in medical sciences : MRMS 11(1), 53-60, 2012-03-01

    Japanese Society for Magnetic Resonance in Medicine

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各種コード

  • NII論文ID(NAID)
    10030284826
  • NII書誌ID(NCID)
    AA11648770
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13473182
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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