Endogenous promoter, 5'-UTR and transcriptional terminator enhance transient gene expression in a liverwort, Marchantia polymorpha L.

この論文にアクセスする

この論文をさがす

著者

抄録

High expression of transgene is one of the key requirements for the successful establishment of transgenic plants to produce a lot of proteins or metabolites. Although the <i>cauliflower mosaic virus 35S</i> (<i>CaMV35S</i>) promoter and the <i>nopaline synthase</i> (<i>NOS</i>) terminator are often used for this purpose, their efficiencies vary to plant species used. In a liverwort, <i>Marchantia polymorpha</i> L., the <i>CaMV35S</i> promoter and the <i>NOS</i> terminator do not show significant enhancing effect of transgene expression. To construct more efficient gene expression system of the liverwort, we employed the transient gene expression assay system. As a result, the endogenous <i>elongation factor 1α</i> promoter and <i>Flowering locus T1</i> terminator from the liverwort led to enhance transient gene expression, approximately 75 and 3 times, respectively, compared to the <i>CaMV35S</i> promoter and the <i>NOS</i> terminator. Furthermore, we found that the endogenous 5′-UTR of the liverwort <i>ADH-like UDP glucose dehydrogenase</i> (<i>MpUDP</i>) yielded an enhancement of 15 times greater than in cases without the <i>MpUDP</i> 5′-UTR. These results indicate that DNA elements enhancing gene expression can be obtained efficiently by the transient gene expression assay in a short period of time, promising the application to the transgene expression system for production of proteins and metabolites in the liverwort.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 28(5), 493-496, 2011-12-25

    日本植物細胞分子生物学会

参考文献:  12件中 1-12件 を表示

各種コード

  • NII論文ID(NAID)
    10030304103
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    023386204
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ