The promoter from tomato sucrose synthase gene TOMSSF drives stamen-specific gene expression in Chrysanthemum

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Anther-specific promoters have been extensively studied in terms of the genetic engineering of male-sterile plants. Here, we fused the 5′ upstream promoter regions of a sucrose synthase gene from tomato (<i>TOMSSF</i>) to the β-glucuronidase (<i>GUS</i>) gene and used the construct to transform <i>Chrysanthemum</i> plants. Histochemical analysis of two transformants showed high GUS activity in ray florets and tubular florets. Analysis of the staining pattern in these florets reveled that staining was stamen-specific. <i>GUS</i> gene expression was highest in the stamen and remained at a steady-state level throughout the experiment. These results suggest that the <i>TOMSSF</i> promoter has high activity in the stamen of tubular florets and would be useful as a high stamen-specific promoter in <i>Chrysanthemum</i>.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 28(5), 497-501, 2011-12-25

    日本植物細胞分子生物学会

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各種コード

  • NII論文ID(NAID)
    10030304116
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    023387597
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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