A versatile method to prevent transcriptional gene silencing in Arabidopsis thaliana

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Transcriptional gene silencing (TGS) is a phenomenon by which transgenes that share homology in their promoter regions are inactivated. TGS is known to be reduced by using different promoters to drive the expression of each transgene. However, in order to perform a large-scale genetic screen in which overexpression lines are mutagenized by T-DNA tagging and therefore harbor two types of transgene, it is critical to develop a technique that consistently blocks TGS. Here, I report a versatile method that completely prevents TGS in transgenic Arabidopsis. A seedling morphology-based assay demonstrated that TGS could be significantly diminished by using different terminator sequences for each transgene. Furthermore, it was suggested that TGS might be reduced if the orientations of the two T-DNA sequences were reversed relative to each other. By combining these strategies, I showed that TGS was completely blocked in over 50,000 T1 plants. These findings present a thorough and versatile method to prevent TGS that is potentially applicable to various plant species and is expected to be used in diverse situations, from basic to applied research fields.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 28(5), 515-519, 2011-12-25

    日本植物細胞分子生物学会

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各種コード

  • NII論文ID(NAID)
    10030304205
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    023387647
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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