GNOM-LIKE1/ERMO1 and SEC24a/ERMO2 Are Required for Maintenance of Endoplasmic Reticulum Morphology in<i>Arabidopsis thaliana</i>

• Ryohei Thomas Nakano

  Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan

• Ryo Matsushima

  Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan

• Haruko Ueda

  Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan

• Kentaro Tamura

  Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan

• Tomoo Shimada

  Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan

• Lixin Li

  Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan

• Yasuko Hayashi

  Department of Environmental Science, Faculty of Science, Niigata University, Niigata 950-2181, Japan

• Maki Kondo

  Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan

• Mikio Nishimura

  Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan

• Ikuko Hara-Nishimura

  Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan

抄録

The endoplasmic reticulum (ER) is composed of tubules, sheets, and three-way junctions, resulting in a highly conserved polygonal network in all eukaryotes. The molecular mechanisms responsible for the organization of these structures are obscure. To identify novel factors responsible for ER morphology, we employed a forward genetic approach using a transgenic Arabidopsis thaliana plant (GFP-h) with fluorescently labeled ER. We isolated two mutants with defects in ER morphology and designated them endoplasmic reticulum morphology1 (ermo1) and ermo2. The cells of both mutants developed a number of ER-derived spherical bodies, ∼1 μm in diameter, in addition to the typical polygonal network of ER. The spherical bodies were distributed throughout the ermo1 cells, while they formed a large aggregate in ermo2 cells. We identified the responsible gene for ermo1 to be GNOM-LIKE1 (GNL1) and the gene for ermo2 to be SEC24a. Homologs of both GNL1 and SEC24a are involved in membrane trafficking between the ER and Golgi in yeast and animal cells. Our findings, however, suggest that GNL1/ERMO1 and SEC24a/ERMO2 have a novel function in ER morphology in higher plants.

収録刊行物

• The Plant Cell

  The Plant Cell 21 (11), 3672-3685, 2009-11-01

  Oxford University Press (OUP)

キーワード

• Cell Biology
• Plant Science

詳細情報

• CRID: 1360846642778280448
• NII論文ID: 10030306087
• DOI: 10.1105/tpc.109.068270
• ISSN: 1532298X
• ISSN: 10404651
• ISSN: http://id.crossref.org/issn/10404651
• Web Site: http://academic.oup.com/plcell/article-pdf/21/11/3672/36924923/plcell_v21_11_3672.pdf
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