凍結包埋剤を用いた簡便な徒手切片法による動物組織とニワトリ胚の観察

• 梶原 裕二

  京都教育大学・生物学教室研究

• 八十田 茂希

  京都教育大学・生物学教室研究

書誌事項

タイトル別名

• Observations of animal tissues and developing chick embryos by newly developed a hand-made preparation with the embedding medium for frozen tissue specimens.
• 凍結包埋材を用いた簡便な徒手切片法による動物組織とニワトリ胚の観察
• トウケツ ホウマイザイ オ モチイタ カンベン ナ トシュ セッペンホウ ニ ヨル ドウブツ ソシキ ト ニワトリハイ ノ カンサツ

抄録

In contrast with the many activities using plant tissues, there have been few experimental studies that observed the animal tissues in the biology class in high schools, and early-grades of college. In the present experiment, a brief and simple method was developed using both an embedding medium for frozen tissue specimens and a broccoli stem as their holder. Before the usage, the water in stems of broccoli were replaced with 20 % ethanol to prevent freezing. Some organs of the mice (C57BL and ICR strains) and chick embryos were fixed with 10 % formalin in phosphate buffered saline. The samples frozen with the embedding medium in the broccoli stem has been sectionned with a razor-blade by hand. The tissue structures of the testis, the kidney, the cerebrum, the cerebellum, the lung and the small intestine, as well as chick embryos could be observed with the slide prepared for a microscope by the present method. Thus, it was shown that the present method was good for the biology class of high schools.

収録刊行物

• 生物教育

  生物教育 52 (3), 112-120, 2011

  一般社団法人 日本生物教育学会

キーワード

• animal tissue
• chick embryo
• frozen-method
• OCT compound

詳細情報

• CRID: 1390564227315817856
• NII論文ID: 10030350086
• NII書誌ID: AN0012957X
• DOI: 10.24718/jjbe.52.3_112
• NDL書誌ID: 023369813
• ISSN: 0287119X
• Web Site: http://id.ndl.go.jp/bib/023369813
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023369813
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可