Application of PTR-MS to an incubation experiment of the marine diatom Thalassiosira pseudonana

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Emission of trace gases from the marine diatom Thalassiosira pseudonana (CCMP 1335) was continuously monitored with a proton transfer reaction-mass spectrometry (PTR-MS) in an axenic batch culture system under a 13:11-h light:dark cycle. Substantial increases in the signals at m/z 49, 63, and 69, attributable to methanethiol, dimethyl sulfide (DMS), and isoprene, respectively, were observed in response to increases in cell density. Signals at m/z 69 showed diurnal variations throughout the experiment whereas those at m/z 49 were more pronounced at the beginning of the incubation. Interestingly, the signals at m/z 49 and 69 changed immediately following the light-dark and dark-light transitions, suggesting that light plays a crucial role in the production of methanethiol and isoprene. However, in the latter half of the experiment, methanethiol showed negligible diurnal variations regardless of light conditions, suggesting the production of methanethiol from enzymatic cleavage of DMS. The trend ill signals at m/z 63 was similar to that of the abundance of senescent cells plus cell debris rather than vegetative cells. The results suggest that aging or death of phytoplankton cells could also substantially control DMS production in natural waters along with the other microbial processes related to bacteria and zooplankton.

Emission of trace gases from the marine diatom <i>Thalassiosira pseudonana</i> (CCMP1335) was continuously monitored with a proton transfer reaction-mass spectrometry (PTR-MS) in an axenic batch culture system under a 13:11-h light:dark cycle. Substantial increases in the signals at <i>m</i>/<i>z</i> 49, 63, and 69, attributable to methanethiol, dimethyl sulfide (DMS), and isoprene, respectively, were observed in response to increases in cell density. Signals at <i>m</i>/<i>z</i> 69 showed diurnal variations throughout the experiment whereas those at <i>m</i>/<i>z</i> 49 were more pronounced at the beginning of the incubation. Interestingly, the signals at <i>m</i>/<i>z</i> 49 and 69 changed immediately following the light-dark and dark-light transitions, suggesting that light plays a crucial role in the production of methanethiol and isoprene. However, in the latter half of the experiment, methanethiol showed negligible diurnal variations regardless of light conditions, suggesting the production of methanethiol from enzymatic cleavage of DMS. The trend in signals at <i>m</i>/<i>z</i> 63 was similar to that of the abundance of senescent cells plus cell debris rather than vegetative cells. The results suggest that aging or death of phytoplankton cells could also substantially control DMS production in natural waters along with the other microbial processes related to bacteria and zooplankton.

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  • Geochemical journal : journal of the Geochemical Society of Japan

    Geochemical journal : journal of the Geochemical Society of Japan 45(5), 355-363, 2011-10-01

    GEOCHEMICAL SOCIETY OF JAPAN

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各種コード

  • NII論文ID(NAID)
    10030362941
  • NII書誌ID(NCID)
    AA00654975
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00167002
  • NDL 記事登録ID
    11288005
  • NDL 雑誌分類
    ZM41(科学技術--地球科学)
  • NDL 請求記号
    Z53-R488
  • データ提供元
    CJP書誌  NDL  IR  J-STAGE 
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