1,2-Di-O-.ALPHA.-linolenoyl-3-O-.BETA.-galactosyl-sn-glycerol as a Superoxide Generation Inhibitor from Perilla frutescens var. crispa

• TAKAHASHI Masakazu

  Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University

• SUGIYAMA Yuta

  Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University

• KAWABATA Kyuichi

  Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University

• TAKAHASHI Yu

  Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University

• IRIE Kazuhiro

  Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University

• MURAKAMI Akira

  Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University

• KUBO Yoshito

  Fukui Prefectural Food Processing Research Institute

• KOBAYASHI Kyoichi

  Fukui Prefectural Food Processing Research Institute

• OHIGASHI Hajime

  Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University

Bibliographic Information

Other Title

• 1,2-Di-O-α-linolenoyl-3-O-β-galactosyl-sn-glycerol as a Superoxide Generation Inhibitor from Perilla frutescens var. crispa
• 1,2-Di-<i>O</i>-α-linolenoyl-3-<i>O</i>-β-galactosyl-<i>sn</i>-glycerol as a Superoxide Generation Inhibitor from<i>Perilla frutescens</i>var.<i>crispa</i>

Abstract

Using a superoxide (O₂⁻) generation assay system with differentiated HL-60 cells, 1,2-di-O-α-linolenoyl-3-O-β-galactosyl-sn-glycerol (DLGG) was identified as an O₂⁻ generation inhibitor from Perilla frutescens var. crispa (a local variety, kida-chirimen shiso). DLGG suppressed the O₂⁻ level in a dose-dependent manner with an IC₅₀ value of 21 μM, comparable to those of rosmarinic acid (RoA, IC₅₀=29 μM) and caffeic acid (CA, IC₅₀=30 μM). While RoA and CA also dose-dependently inhibited O₂⁻ generation in a xanthine-xanthine oxidase system, DLGG had no effect in the same system. Thus DLGG appeared to decrease the O₂⁻ level in the HL-60 assay system by mechanisms different from those of RoA and CA, which appeared to act as O₂⁻ scavengers.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 75 (11), 2240-2242, 2011

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• <I>Perilla frutescens</I>
• 1,2-di-<I>O</I>-α-linolenoyl-3-<I>O</I>-β-galactosyl-<I>sn</I>-glycerol (DLGG)
• superoxide
• xanthine oxidase
• HL-60 cells

Details

• CRID: 1390001206478209024
• NII Article ID: 10030397123
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.110414
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 023331751
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023331751
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110414
• Web Site: https://search.jamas.or.jp/link/ui/2012139882
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed