A Comparative Analysis of the Molecular Characteristics of the Arabidopsis CoA Pyrophosphohydrolases AtNUDX11, 15, and 15a
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- ITO Daisuke
- Department of Advanced Bioscience, Faculty of Agriculture, Kinki University
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- YOSHIMURA Kazuya
- Department of Food and Nutritional Science, College of Bioscience and Biotechnology, Chubu University
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- ISHIKAWA Kazuya
- Department of Advanced Bioscience, Faculty of Agriculture, Kinki University
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- OGAWA Takahisa
- Department of Advanced Bioscience, Faculty of Agriculture, Kinki University
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- MARUTA Takanori
- Department of Advanced Bioscience, Faculty of Agriculture, Kinki University
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- SHIGEOKA Shigeru
- Department of Advanced Bioscience, Faculty of Agriculture, Kinki University
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抄録
Coenzyme A (CoA) is an essential, ubiquitous cofactor in all biological systems, where it acts as the major acyl group carrier in various central metabolic reactions. Although much is known about CoA biosynthesis, it is unclear how the CoA pool is regulated the various cellular compartments. It has been found that the nucleoside diphosphates linked to some moiety X (Nudix) hydrolases, AtNUDX11 and 15, have pyrophosphohydrolase activity toward CoA and its derivatives. In this study we identified two alternatively spliced variants, AtNUDX15 and 15a, produced from the AtNUDX15 gene, and carried out comparative studies of the gene regulation, the kinetic parameters, and the intracellular localization of AtNUDX11, 15, and 15a. The present findings indicate that AtNUDX11 and AtNUDX15(a) function in the hydrolysis of malonyl-CoA in cytosol and succinyl-CoA in the mitochondria, respectively, suggesting their impact not only on CoA biosynthesis but also on various CoA-related pathways such as the TCA cycle.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 76 (1), 139-147, 2012
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206478496896
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- NII論文ID
- 10030398798
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 023407770
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 使用不可