Pyrrolysine Analogs as Substrates for Bacterial Pyrrolysyl-tRNA Synthetase in Vitro and in Vivo

• KATAYAMA Hidekazu

  Department of Applied Biochemistry, School of Engineering, Tokai University

• NOZAWA Kayo

  Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo

• NUREKI Osamu

  Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo

• NAKAHARA Yoshiaki

  Department of Applied Biochemistry, School of Engineering, Tokai University

• HOJO Hironobu

  Department of Applied Biochemistry, School of Engineering, Tokai University Institute of Glycoscience, Tokai University

Bibliographic Information

Other Title

• Pyrrolysine Analogs as Substrates for Bacterial Pyrrolysyl-tRNA Synthetase<i>in Vitro</i>and<i>in Vivo</i>

Abstract

Pyrrolysine-tRNA^Pyl complex is produced by pyrrolysyl-tRNA synthetase (PylRS). In this study, we investigated the substrate specificity of Desulfitobacterium hafnience PylRS. PylRS incorporated various L-lysine derivatives into tRNA^Pyl in vitro. In addition, the PylRS/tRNA^Pyl pair introduced these lysine derivatives into the recombinant protein by the Escherichia coli expression system, indicating that this PylRS/tRNA^Pyl pair can be used in protein engineering technology.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 76 (1), 205-208, 2012

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• <I>Desulfitobacterium hafnience</I>
• pyrrolysine
• pyrrolysyl-tRNA synthetase

Details

• CRID: 1390001206478457984
• NII Article ID: 10030399195
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.110653
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 023408115
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023408115
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110653
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed