Temperature-Dependent Self-Splicing Group I Introns in the Flagellin Genes of the Thermophilic Bacillus Species

• HAYAKAWA Jumpei

  Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University

• ISHIZUKA Morio

  Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University

Bibliographic Information

Other Title

• Temperature-Dependent Self-Splicing Group I Introns in the Flagellin Genes of the Thermophilic<i>Bacillus</i>Species

Abstract

A previous report described the presence of a self-splicing group I intron in a flagellin gene from a thermophilic Bacillus species. Here, we present evidence that the splicing reaction of the flagellin introns is dependent on temperature. Furthermore, a complementation analysis using a Bacillus subtilis flagellin-deficient mutant indicated that the intron-containing flagellin gene significantly restored the motility of the mutant at higher temperatures.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 76 (2), 410-413, 2012

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• ribozyme
• group I self-splicing intron
• flagellin
• bacterial intron
• thermophilic <I>Bacillus</I>

Details

• CRID: 1390001206479764096
• NII Article ID: 10030400412
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.110741
• COI: 1:STN:280:DC%2BC383lvV2huw%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 023465473
• PubMed: 22313782
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023465473
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110741
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed