Expression and Localization of Two SecA Homologs in the Unicellular Red Alga Cyanidioschyzon merolae

Access this Article

Search this Article

Author(s)

    • MATSUMOTO Kouji
    • Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University
    • HARA Hiroshi
    • Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University
    • OHTA Niji
    • Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University

Abstract

SecA is an ATP-driven motor for Sec translocase that participates in bacterial protein export and thylakoidal import in plants. We have reported that <I>Cyanidioschyzon merolae</I>, a unicellular red alga, possesses a nuclear-encoded <I>secA</I>(nuc) and a plastid-encoded <I>secA</I>(pt) gene. In this study we found that the amount of SecA(nuc) protein almost quadrupled at high temperature, whereas that of the SecA(pt) protein increased far less. We were also able to determine the localization of both SecAs to the chloroplast by immunofluorescence and immunoelectron microscopy. We suggest that SecA(nuc) has an important role in the chloroplast at high temperatures.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 76(2), 417-422, 2012-02-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  16

Codes

  • NII Article ID (NAID)
    10030400439
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    SHO
  • ISSN
    09168451
  • NDL Article ID
    023465526
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  NDL  J-STAGE 
Page Top