Role of Acidification Elicited by Sialylation and Sulfation of Zona Glycoproteins During Oocyte Maturation in Porcine Sperm-zona Pellucida Interactions
-
- LAY Khin Mar
- Science of Bioresource Production, The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan
-
- OSHIRO Ryuko
- Faculty of Agriculture, University of the Ryukyus, Okinawa 903-0213, Japan
-
- ARASAKI Chiemi
- Faculty of Agriculture, University of the Ryukyus, Okinawa 903-0213, Japan
-
- ASHIZAWA Koji
- Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan
-
- TATEMOTO Hideki
- Faculty of Agriculture, University of the Ryukyus, Okinawa 903-0213, Japan
Search this article
Abstract
The porcine zona pellucida (ZP) undergoes biochemical changes during the final phase of maturation prior to fertilization. The present study was conducted to elucidate whether the acidification of ZP glycoproteins during porcine oocyte maturation influences sperm-ZP interactions. Two-dimensional gel electrophoresis clearly demonstrated that ZP acidification occurred in accordance with the sialylation and sulfation of ZP glycoproteins in oocytes matured for 44 h. The increases in the incidences of sperm penetration and polyspermy with the progress of the IVM culture period were significantly suppressed by ZP desialylation on treatment with neuraminidase as a consequence of reductions in the number of sperm bound to ZPs and the acrosome reaction (AR) in ZP-bound sperm (P<0.05). In contrast, the blocking of ZP sulfation by NaClO3 treatment during IVM markedly reduced the incidence of polyspermy with no inhibitory effect on penetration, but the number of sperm bound to ZPs and the rate of AR-inducing sperm were decreased to the same level as in desialylated oocytes. The results indicate that ZP sulfation influences sperm-ZP interactions in a ZP sialylation-independent manner. Moreover, sialylation and sulfation were not associated with a protective proteolytic modification of the ZP matrix before fertilization. These findings suggest that ZP acidification elicited by the sialylation and sulfation of ZP glycoproteins during oocyte maturation contributes to the porcine ZP acquiring the capacity to accept sperm.
Journal
-
- Journal of Reproduction and Development
-
Journal of Reproduction and Development 57 (6), 744-751, 2011
The Society for Reproduction and Development
- Tweet
Details 詳細情報について
-
- CRID
- 1390001206336890496
-
- NII Article ID
- 10030406421
-
- NII Book ID
- AA10936678
-
- ISSN
- 13484400
- 09168818
-
- HANDLE
- 10458/4280
-
- NDL BIB ID
- 023388302
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed