Construction of Designed Peptide Microarrays Toward "Omics" Studies

• USUI Kenji

  FIRST (Faculty of Frontiers of Innovative Research in Science and Technology) and FIBER (Frontier Institute for Biomolecular Engineering Research), Konan University

• TOMIZAKI Kin-ya

  Innovative Materials and Processing Research Center and Department of Materials Chemistry, Ryukoku University

• MIHARA Hisakazu

  Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology

Bibliographic Information

Other Title

• "オミクス"研究に向けた設計ペプチドアレイの構築
• "オミクス"ケンキュウ ニ ムケタ セッケイ ペプチドアレイ ノ コウチク

Abstract

Array-based technologies, including DNA microarrays and protein-detecting microarrays, have become more attractive as promising high-throughput research tools in "omics" studies. So far, we have designed peptide libraries composed of various secondary structures to be used for microarray technologies, especially in proteome fields and cellomics fields. Here, we describe the development of microarrays using designed peptides toward "omics" studies including genomics, proteomics, glycomics and cellomics. Designed peptides are potent capturing agents for microarray technology because of the following advantages : 1) Peptides are easier to design and synthesize in a short sequence mimicking other complicated biomolecules. 2) Antimicrobial, signaling, and/or transmembrane activities of peptides make them promising control tools for cellular events. Moreover, novel methods for detection technology, which is one of the key technologies for microarrays, were also developed. New fluorescent-label methods utilizing the excluded volume effect of the target molecule or fluorometric/colorimetric change of the detection moiety were achieved. A useful label-free method utilizing an AR (anomalous reflection of gold) system was also constructed and improved. A microarray using designed peptides would be one of the most promising tools for high-throughput and detailed analyses in "omics" researches.

Journal

• BUNSEKI KAGAKU

  BUNSEKI KAGAKU 61 (6), 523-534, 2012

  The Japan Society for Analytical Chemistry

Keywords

• microarray
• designed peptide
• detection method
• protein analysis
• cell analysis

Details

• CRID: 1390282679637971328
• NII Article ID: 10030436925
• NII Book ID: AN00222633
• DOI: 10.2116/bunsekikagaku.61.523
• COI: 1:CAS:528:DC%2BC38XhtVylsr3J
• NDL BIB ID: 023832988
• ISSN: 05251931
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023832988
• Web Site: https://www.jstage.jst.go.jp/article/bunsekikagaku/61/6/61_523/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed